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Structured Review

Proteintech anti nrf2
Anti Nrf2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+nrf2/LC3+Antibody/pm42790822-87-25-27
Average 96 stars, based on 2093 article reviews
anti nrf2 - by Bioz Stars, 2026-10
96/100 stars

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Related Articles

Blocking Assay:

Article Title: Study on the potential mechanism of Artemisia integrifolia Linn. in diabetic nephropathy: multiscale analysis of network pharmacology and metabolomics.
Article Snippet: Ethnopharmacological relevance: Artemisia integrifolia Linn. (Chinese name: Liuhao, LH) is a traditional Chinese medicine commonly employed for managing diabetes.. However, its pharmacological effects on diabetic nephropathy (DN) remain unclear.. Aim of the study: This study aimed to investigate the effects and underlying mechanisms of LH on DN.

Incubation:

Article Title: Study on the potential mechanism of Artemisia integrifolia Linn. in diabetic nephropathy: multiscale analysis of network pharmacology and metabolomics.
Article Snippet: Ethnopharmacological relevance: Artemisia integrifolia Linn. (Chinese name: Liuhao, LH) is a traditional Chinese medicine commonly employed for managing diabetes.. However, its pharmacological effects on diabetic nephropathy (DN) remain unclear.. Aim of the study: This study aimed to investigate the effects and underlying mechanisms of LH on DN.

Article Title: Organic copper substitution mitigates feed-derived copper emissions by enhancing bioefficacy and redox–microbiota homeostasis in Eriocheir sinensis
Article Snippet: For protein analysis, hepatopancreas tissues (four samples per group) were lysed with RIPA buffer (Beyotime, Shanghai, China), and total protein was quantified via BCA assay. .. Samples were separated by SDS-PAGE and transferred to PVDF membranes (Merck, Germany), blocked with 5% non-fat milk, and incubated with primary antibodies (anti-NRF2, 1:1000, Proteintech, Wuhan, China; anti-SOD, 1:1000, Sigma-Aldrich, USA), followed by HRP-conjugated secondary antibodies (1:5000, Proteintech). .. Signals were visualized with ECL reagents and imaged on an ImageQuant LAS 4000 system (GE Healthcare, Chicago, USA).

Cell-Signaling:

Article Title: Kuwanon E sensitizes hepatocellular carcinoma cells to disulfidptosis via MTOR-mediated autophagy blockade.
Article Snippet: Disulfidptosis, a recently identified form of regulated cell death driven by toxic intracellular disulfide accumulation and subsequent collapse of the actin cytoskeleton, represents a promising therapeutic vulnerability in hepatocellular carcinoma (HCC), particularly for tumors exhibiting metabolic reprogramming and resistance to conventional apoptosis-based therapies.. In this study, we screened eleven representative bioactive constituents derived from Cortex Mori, including stilbenoids, flavonoids, coumarins, and phenolic acids, under glucose deprivation conditions that trigger disulfidptosis, and identified Kuwanon E (KE) as the most potent sensitizer of disulfidptosis in HCC cells.. Integrative network pharmacology, RNA sequencing, and untargeted metabolomics revealed that KE preferentially targets the MTOR signaling pathway and autophagic flux.

Article Title: MLKL confers ferroptosis resistance in colorectal cancer cells via impaired ESCRT-III-mediated membrane repair
Article Snippet: Chloroquine, Deferoxamine, Ferrostatin-1, Liproxstatin-1, ML210, and RSL3 were purchased from APExBIO (Houston, TX, USA), while zVAD-fmk was obtained from Merck Millipore (Darmstadt, Germany). .. Antibodies used for Western blot and immunofluorescence were purchased from the following sources: anti-GPX4 (ab125066), anti-HMGB1 (ab79823) and anti-MLKL (ab194699) from Abcam (Cambridge, UK); anti-β-actin (3700), anti-LC3B (2775) and anti-SLC7A11 (12691) from Cell Signaling Technology (Danvers, MA, USA); anti-KEAP1 (10503-2-AP) and anti-NRF2 (16396-1-AP) from Proteintech (Rosemont, IL, USA) and anti-CHMP5 (sc-374337) from Santa Cruz Biotechnology (Dallas, TX, USA). .. HT-29 (RRID: CVCL_0320), SW620 (RRID: CVCL_0547), A549 (RRID: CVCL_0023), and MDA-MB-231 (RRID: CVCL_0062) cell lines were obtained from the American Type Culture Collection (ATCC).

Western Blot:

Article Title: MLKL confers ferroptosis resistance in colorectal cancer cells via impaired ESCRT-III-mediated membrane repair
Article Snippet: Chloroquine, Deferoxamine, Ferrostatin-1, Liproxstatin-1, ML210, and RSL3 were purchased from APExBIO (Houston, TX, USA), while zVAD-fmk was obtained from Merck Millipore (Darmstadt, Germany). .. Antibodies used for Western blot and immunofluorescence were purchased from the following sources: anti-GPX4 (ab125066), anti-HMGB1 (ab79823) and anti-MLKL (ab194699) from Abcam (Cambridge, UK); anti-β-actin (3700), anti-LC3B (2775) and anti-SLC7A11 (12691) from Cell Signaling Technology (Danvers, MA, USA); anti-KEAP1 (10503-2-AP) and anti-NRF2 (16396-1-AP) from Proteintech (Rosemont, IL, USA) and anti-CHMP5 (sc-374337) from Santa Cruz Biotechnology (Dallas, TX, USA). .. HT-29 (RRID: CVCL_0320), SW620 (RRID: CVCL_0547), A549 (RRID: CVCL_0023), and MDA-MB-231 (RRID: CVCL_0062) cell lines were obtained from the American Type Culture Collection (ATCC).

Immunofluorescence:

Article Title: MLKL confers ferroptosis resistance in colorectal cancer cells via impaired ESCRT-III-mediated membrane repair
Article Snippet: Chloroquine, Deferoxamine, Ferrostatin-1, Liproxstatin-1, ML210, and RSL3 were purchased from APExBIO (Houston, TX, USA), while zVAD-fmk was obtained from Merck Millipore (Darmstadt, Germany). .. Antibodies used for Western blot and immunofluorescence were purchased from the following sources: anti-GPX4 (ab125066), anti-HMGB1 (ab79823) and anti-MLKL (ab194699) from Abcam (Cambridge, UK); anti-β-actin (3700), anti-LC3B (2775) and anti-SLC7A11 (12691) from Cell Signaling Technology (Danvers, MA, USA); anti-KEAP1 (10503-2-AP) and anti-NRF2 (16396-1-AP) from Proteintech (Rosemont, IL, USA) and anti-CHMP5 (sc-374337) from Santa Cruz Biotechnology (Dallas, TX, USA). .. HT-29 (RRID: CVCL_0320), SW620 (RRID: CVCL_0547), A549 (RRID: CVCL_0023), and MDA-MB-231 (RRID: CVCL_0062) cell lines were obtained from the American Type Culture Collection (ATCC).

SDS Page:

Article Title: Organic copper substitution mitigates feed-derived copper emissions by enhancing bioefficacy and redox–microbiota homeostasis in Eriocheir sinensis
Article Snippet: For protein analysis, hepatopancreas tissues (four samples per group) were lysed with RIPA buffer (Beyotime, Shanghai, China), and total protein was quantified via BCA assay. .. Samples were separated by SDS-PAGE and transferred to PVDF membranes (Merck, Germany), blocked with 5% non-fat milk, and incubated with primary antibodies (anti-NRF2, 1:1000, Proteintech, Wuhan, China; anti-SOD, 1:1000, Sigma-Aldrich, USA), followed by HRP-conjugated secondary antibodies (1:5000, Proteintech). .. Signals were visualized with ECL reagents and imaged on an ImageQuant LAS 4000 system (GE Healthcare, Chicago, USA).



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Image Search Results


Journal: iScience

Article Title: Pentachlorophenol enhances bladder cancer cell invasion through altered protein stability

doi: 10.1016/j.isci.2026.117357

Figure Lengend Snippet:

Article Snippet: Rabbit anti-NFE2L2 , ABclonal , Cat# A3577; RRID: AB_2863473.

Techniques: Recombinant, CCK-8 Assay, Bicinchoninic Acid Protein Assay, shRNA, Plasmid Preparation, Over Expression, Software, Transfection, Western Blot